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Prospective evaluation of the Septi FAST multiplex real-time PCR assay for surveillance and diagnosis of infections in haematological patients after allogeneic stem cell transplantation compared to routine microbiological assays and an in-house real-time PCR method
- Source :
- Mycoses; Dec2017, Vol. 60 Issue 12, p781-788, 8p
- Publication Year :
- 2017
-
Abstract
- We prospectively evaluated a multiplex real-time PCR assay (SeptiFast, SF) in a cohort of patients undergoing allo- BMT in comparison to an in-house PCR method ( IH- PCR). Overall 847 blood samples (mean 8 samples/patient) from 104 patients with haematological malignancies were analysed. The majority of patients had acute leukaemia (62%) with a mean age of 52 years (54% female). Pathogens could be detected in 91 of 847 (11%) samples by SF compared to 38 of 205 (18.5%) samples by BC, and 57 of 847 (6.7%) samples by IH- PCR. Coagulase-negative staphylococci (n=41 in SF, n=29 in BC) were the most frequently detected bacteria followed by Escherichia coli (n=9 in SF, n=6 in BC). Candida albicans (n=17 in SF, n=0 in BC, n=24 in IH- PCR) was the most frequently detected fungal pathogen. SF gave positive results in 5% of samples during surveillance vs in 26% of samples during fever episodes. Overall, the majority of blood samples gave negative results in both PCR methods resulting in 93% overall agreement resulting in a negative predictive value of 0.96 (95% CI: 0.95-0.97), and a positive predictive value of 0.10 (95% CI: −0.01 to 0.21). SeptiFast appeared to be superior over BC and the IH- PCR method. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09337407
- Volume :
- 60
- Issue :
- 12
- Database :
- Complementary Index
- Journal :
- Mycoses
- Publication Type :
- Academic Journal
- Accession number :
- 126407448
- Full Text :
- https://doi.org/10.1111/myc.12662