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Fibrin-Enhanced Canonical Wnt Signaling Directs Plasminogen Expression in Cementoblasts.
- Source :
- International Journal of Molecular Sciences; Nov2017, Vol. 18 Issue 11, p2380, 19p
- Publication Year :
- 2017
-
Abstract
- OCCM30-fibrin showed higher levels of Wnt3a expression, nuclear translocation of β-catenin, and T-cell factor (TCF) optimal motif (TOP) reporter activity than the cells on tissue culture dishes (OCCM30-TCD), indicating that the OCCM30-fibrin enhanced canonical Wnt/β-catenin signaling. Also, OCCM30-fibrin expressed biomineralization-associated markers at higher levels than OCCM30-TCD, of which levels were further increased with LiCl, a Wnt signaling activator. The OCCM30 cementoblasts simultaneously showed that high levels of plasminogen, a critical component of fibrinolysis, were expressed in the OCCM30-fibrin. Activation of canonical Wnt signaling with LiCl treatment or with forced lymphoid enhancer factor 1 (LEF1)-expression increased the expression of plasminogen. On the contrary, the inhibition of canonical Wnt signaling with siRNAs against Wnt3a or β-catenin abrogated fibrin-enhanced plasminogen expression. Furthermore, there are three conserved putative response elements for the LEF1/β-catenin complex in the plasminogen proximal promoter regions (􀀀900 to +54). Site-directed mutations and chromatin immunoprecipitation indicated that canonical Wnt signaling directed plasminogen expression. Taken together, this study suggests that fibrin-based materials can modulate functional periodontal formations in controlling cementoblast differentiation and fibrin degradation. [ABSTRACT FROM AUTHOR]
- Subjects :
- PLASMINOGEN
CELLS
CATENINS
T cells
BIOMINERALIZATION
Subjects
Details
- Language :
- English
- ISSN :
- 16616596
- Volume :
- 18
- Issue :
- 11
- Database :
- Complementary Index
- Journal :
- International Journal of Molecular Sciences
- Publication Type :
- Academic Journal
- Accession number :
- 126421297
- Full Text :
- https://doi.org/10.3390/ijms18112380