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An electrochemical microRNA sensing platform based on tungsten diselenide nanosheets and competitive RNA–RNA hybridization.
- Source :
- Analyst; 12/21/2017, Vol. 142 Issue 24, p4843-4851, 9p
- Publication Year :
- 2017
-
Abstract
- In this work, we report an ultrasensitive electrochemical biosensor for microRNA-21 (miRNA-21) detection by using a competitive RNA–RNA hybridization configuration. A biotinylated miRNA of the self-same sequence with the target miRNA is mixed with the samples, and allowed competition with the target miRNA for a thiolated RNA probe immobilized onto a tungsten diselenide (WSe<subscript>2</subscript>) nanosheet modified electrode. Thereafter the current response is obtained by forming the hybridized biotinylated miRNA with streptavidin–horseradish peroxidase (HRP) conjugates to catalyze the H<subscript>2</subscript>O<subscript>2</subscript> + hydroquinone (HQ) system. Benefiting from the high specific surface area of WSe<subscript>2</subscript> nanosheets, the competitive hybridization configuration and the signal amplification of the H<subscript>2</subscript>O<subscript>2</subscript> + HQ detection system, the proposed assay exhibits a wide linear range of 0.0001–100 pM towards target miRNA with a detection limit of 0.06 fM (S/N = 3), and shows excellent discrimination ability for base-mismatched miRNA sequences. Therefore, the designed platform has promising prospects for the detection of miRNA in biomedical research and early clinical diagnosis. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00032654
- Volume :
- 142
- Issue :
- 24
- Database :
- Complementary Index
- Journal :
- Analyst
- Publication Type :
- Academic Journal
- Accession number :
- 126553630
- Full Text :
- https://doi.org/10.1039/c7an01244f