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Stability Indicating HPLC-UV vs. UPLC-DAD for Estimation of Atorvastatin Simultaneously with Aspirin, Clopidogrel and their Related Impurities in Bulk and Capsules.
- Source :
- Analytical Chemistry Letters; Oct2017, Vol. 7 Issue 5, p596-610, 15p
- Publication Year :
- 2017
-
Abstract
- Aspirin (ASP) (analgesic; anti-inflammatory; anti-platelet), clopidogrel bisulphate (CLP) (anti-platelet) and atorvastatin calcium (ATV) (anti-hyperlipidemia) are co-formulated in several pharmaceutical formulations. Salicylic acid (SAL), clopidogrel carboxylic acid metabolite related impurity A (CLP-RA) and clopidogrel inactiveR-enantiomer related impurity C (CLP-RC) are considered to be the main synthesis impurities and degradation products of ASP and clopidogrel. On the other hand, high efficiency and less elution are the basic requirements of high-speed chromatographic separation. So a comparative study of two simple, precise, and accurate reversed-phase HPLC-UV and UPLC-DAD methods were developed and validated for simultaneous estimation of ASP, CLP and ATV in bulk and capsules and in the presence of SAL, CLP-RA and CLP-RC. An Agilent Eclipse plus C18 (250 mm × 4.6 mm, 5 μm) for HPLC method and an Agilent Eclipse plus C18 (100 × 2.1 mm, 1.7 μm) for UPLC method were used. A gradient mobile phase for HPLC method and an isocratic mobile phase for UPLC, were used. Detection was performed for both methods at 220 nm for ASP, CLP, SAL, CLP-RA and CLP-RC, and at 240 nm for ATV. The propsed UPLC-DAD method had advantages, of being economic on the large scale with relatively much satisfactory results for sensitivity and specificity. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 22297928
- Volume :
- 7
- Issue :
- 5
- Database :
- Complementary Index
- Journal :
- Analytical Chemistry Letters
- Publication Type :
- Academic Journal
- Accession number :
- 126729837
- Full Text :
- https://doi.org/10.1080/22297928.2017.1385418