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Determination of dihydromyricetin in rat plasma by LC-MS/MS and its application to a pharmacokinetic study.
- Source :
- Pharmaceutical Biology; Dec2017, Vol. 55 Issue 1, p657-662, 6p
- Publication Year :
- 2017
-
Abstract
- Context:The pharmacokinetics properties of dihydromyricetin (DHM) are still unknown. Objective:This study investigates the pharmacokinetic characteristics of DHM using a sensitive and reliable LC-MS/MS method. Materials and methods:A rapid and sensitive LC-MS/MS method was developed for the determination of DHM in male Sprague–Dawley rat plasma. Twelve rats were equally randomized into two groups, including the intravenous group (2 mg/kg) and the oral group (20 mg/kg). Blood samples (250 μL) were collected at designated time points and analyzed using this method. The pharmacokinetic parameters were calculated using DAS 3.0 pharmacokinetic software. Results:The calibration curve was linear within the range of 0.5–200 ng/mL (r > 0.998) with the lower limit of quantification at 0.5 ng/mL. After the intravenous injection, DHM reached a maximum concentration of 165.67 ± 16.35 ng/mL, andt1/2was 2.05 ± 0.52 h. However, DHM was not readily absorbed and reachedCmax21.63 ± 3.62 ng/mL at approximately 2.67 h following the oral administration of DHM, andt1/2was 3.70 ± 0.99 h. The MRT for the intravenous group and the oral group were 2.62 ± 0.36 and 5.98 ± 0.58 h, respectively. TheAUC(0-t)for the intravenous group and the oral group were 410.73 ± 78.12 and 164.97 ± 41.76 ng·L/mL, respectively, so the absolute bioavailability of DHM was 4.02% which was poor. Discussion and conclusion:The results indicated that the bioavailability was poor. Further work needs to be conducted to investigate the reason for poor bioavailability and improve this situation. [ABSTRACT FROM AUTHOR]
- Subjects :
- FLAVONOIDS
PHARMACOKINETICS
BIOAVAILABILITY
ABSORPTION
AMPELOPSIS
Subjects
Details
- Language :
- English
- ISSN :
- 13880209
- Volume :
- 55
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Pharmaceutical Biology
- Publication Type :
- Academic Journal
- Accession number :
- 126815666
- Full Text :
- https://doi.org/10.1080/13880209.2016.1266669