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Fluorescence-activated droplet sorting of lipolytic microorganisms using a compact optical system.
- Source :
- Lab on a Chip; 1/7/2018, Vol. 18 Issue 1, p190-196, 7p
- Publication Year :
- 2018
-
Abstract
- Lipases are ubiquitous enzymes of great physiological significance that have been used extensively in multiple industries. Environmental microorganisms are a major source for the discovery of novel lipases with high catalytic efficiency and selectivity. However, current plate-based screening of lipase-producing strains is time consuming, labour intensive and inefficient. In this study, we developed an ultra-high throughput screening pipeline for lipase-producing strains based on fluorescence-activated droplet sorting (FADS) using a compact optical system that could be easily set up in an alignment-free manner. The pipeline includes droplet generation, droplet incubation, picoinjection of the fluorescence probe, and sorting of droplets with a throughput of 2 × 10<superscript>6</superscript> drops per h. We applied the pipeline to screen samples collected from different locations, including sediments from a hot spring in Tibet, soils from the Zoige wetland, contaminated soils from an abandoned oilfield, and a Chinese Daqu starter. In total, we obtained 47 lipase-producing bacterial strains belonging to seven genera, including Staphylococcus, Bacillus, Enterobacter, Serratia, Prolinoborus, Acinetobacter, and Leclercia. We believe that this FADS-based pipeline could be extended to screen various enzymes from the environment, and may find wide applications in breeding of industrial microorganisms. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 14730197
- Volume :
- 18
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Lab on a Chip
- Publication Type :
- Academic Journal
- Accession number :
- 126866620
- Full Text :
- https://doi.org/10.1039/c7lc00993c