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Homeostatic 'bystander' proliferation of human peripheral blood B cells in response to polyclonal T-cell stimulation in vitro.
- Source :
- International Immunology; Nov2015, Vol. 27 Issue 11, p579-588, 10p
- Publication Year :
- 2015
-
Abstract
- The mechanisms of maintenance of adequate numbers of B lymphocytes and of protective levels of immunoglobulins in the absence of antigenic (re)stimulation remain not fully understood. Meanwhile, our results presented here show that both peripheral blood naive and memory B cells can be activated strongly and non-specifically (in a mitogen-like fashion) in 5-day in vitro cultures of anti-CD3- or concanavalin A (Con A)-stimulated peripheral blood mononuclear cells of healthy people. This polyclonal, bystander activation of the B cells includes multiple divisions of most of them (assessed here by the flow cytometric technique of dividing cell tracking) and significant antibody [immunoglobulin M (IgM) and IgG] secretion. Observed proliferation of the CD19<superscript>+</superscript> B cells depends on contact with stimulated T helper (T<subscript>h</subscript>) cells (via CD40-CD40L interaction) and on the response of B cells to secreted interleukins IL-5, IL-10 and IL-4, and is correlated with the levels of these T<subscript>h</subscript>-derived molecules, while it does not involve the ligation of the BCR/CD19 complex. We suggest that the effect might reflect the situation occurring in vivo as the homeostatic proliferation of otherwise non-stimulated, peripheral B lymphocytes, providing an always ready pool for efficient antibody production to any new (or cognate) antigen challenge. [ABSTRACT FROM AUTHOR]
- Subjects :
- CELL proliferation
B cells
T cells
IMMUNOGLOBULINS
CONCANAVALIN A
Subjects
Details
- Language :
- English
- ISSN :
- 09538178
- Volume :
- 27
- Issue :
- 11
- Database :
- Complementary Index
- Journal :
- International Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 126965504
- Full Text :
- https://doi.org/10.1093/intimm/dxv032