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In vitro study on the inhibition of enzymatic systems in Italian and Carniolan honey bees by piperonyl butoxide new derivatives.

Authors :
TODESCHINI, Valeria
PANINI, Michela
CHIESA, Olga
PUGGIONI, Vincenzo
ANACLERIO, Matteo
VINŠOVÁ, Hana
KAMLER, Martin
TITĚRA, Dalibor
MAZZONI, Emanuele
Source :
Bulletin of Insectology; 2017, Vol. 70 Issue 2, p237-244, 8p
Publication Year :
2017

Abstract

In this study we measured in vitro honey bee enzyme activities in presence of the synergist piperonyl butoxide (PBO) and some new benzodioxole and dihydrobenzofuran derivatives through absorbance and fluorescence spectrophotometric assays, to determine if and to what extent detoxification systems and acetylcholinesterases are affected by these compounds. Both Italian (Italy) and Carniolan (Czech Republic) honey bees were tested. In the case of Italian honey bees the esterase activity was partially but significantly inhibited by almost all the tested products. In Carniolan honey bees only the dihydrobenzofuran derivative EN16-41 significantly inhibited esterases. Other enzymatic systems, such as cytochrome P450 monooxygenases (P450s) and glutathione S-transferases (GSTs), often involved in xenobiotics detoxification, as well as acetylcholinesterases (AchEs) seem not to be targeted by the studied synergists. These data seem to suggest that some of the investigated PBO analogues could not be detrimental to honey bees. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
17218861
Volume :
70
Issue :
2
Database :
Complementary Index
Journal :
Bulletin of Insectology
Publication Type :
Academic Journal
Accession number :
127393738