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Therapeutic Effects of FK506 on IgA Nephropathy Rat.

Authors :
Wei, Linting
Du, Yan
Jia, Lining
Ma, Xiaotao
Chen, Zhao
Lu, Jiamei
Tian, Lifang
Duan, Zhaoyang
Dong, Fengming
Lv, Zhian
Yao, Ganglian
Fu, Rongguo
Wang, Li
Source :
Kidney & Blood Pressure Research; Jan2018, Vol. 42 Issue 6, p983-998, 16p
Publication Year :
2018

Abstract

Background/Aims: FK506 is an immunosuppressive drug and a calcineurin inhibitor that has been widely used in kidney disease in recent years. FK506 shows a wide range of biological and pharmaceutical effects; however, the mechanism of its anti- proliferative effect has not been well elucidated. An IgA nephropathy (IgAN) model was used to generate a mesangial cell proliferation model. This study aims to examine the effect of FK506 on IgAN rats and the underlying mechanisms. Methods: Hematuria, proteinuria and renal function were measured. To observe the pathological conditions, we performed HE (hematoxylin - eosin) and PAS (periodic acid - schiff) staining. Transcription and protein expression levels were detected by qRT - PCR (quantitative real-time polymerase chain reaction) and Wb (western blotting). The location and semi-quantitative expression levels of TRPCs, CaN (Calcineurin) and α-SMA were examined by IHC (Immunohistochemical staining). Results: We found that FK506 could improve hematuria, proteinuria and renal function, especially in the HF (high-dose FK506) groups. Renal pathological changes were ameliorated in the treatment groups. FK506 could significantly decrease TRPCs, CaN, phosphorylation of ERK1/2 and α-SMA expression. Conclusion: Taken together, these results suggest that the therapeutic effect of FK506 on IgAN might be partially associated with the down-regulated expression of TRPC channels, CaN and phosphorylation of ERK1/2. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
14204096
Volume :
42
Issue :
6
Database :
Complementary Index
Journal :
Kidney & Blood Pressure Research
Publication Type :
Academic Journal
Accession number :
127591911
Full Text :
https://doi.org/10.1159/000485346