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Dual light‐emitting diode‐based multichannel microscopy for whole‐slide multiplane, multispectral and phase imaging.
- Source :
- Journal of Biophotonics; Feb2018, Vol. 11 Issue 2, p1-1, 9p
- Publication Year :
- 2018
-
Abstract
- We report the development of a multichannel microscopy for whole‐slide multiplane, multispectral and phase imaging. We use trinocular heads to split the beam path into 6 independent channels and employ a camera array for parallel data acquisition, achieving a maximum data throughput of approximately 1 gigapixel per second. To perform single‐frame rapid autofocusing, we place 2 near‐infrared light‐emitting diodes (LEDs) at the back focal plane of the condenser lens to illuminate the sample from 2 different incident angles. A hot mirror is used to direct the near‐infrared light to an autofocusing camera. For multiplane whole‐slide imaging (WSI), we acquire 6 different focal planes of a thick specimen simultaneously. For multispectral WSI, we relay the 6 independent image planes to the same focal position and simultaneously acquire information at 6 spectral bands. For whole‐slide phase imaging, we acquire images at 3 focal positions simultaneously and use the transport‐of‐intensity equation to recover the phase information. We also provide an open‐source design to further increase the number of channels from 6 to 15. The reported platform provides a simple solution for multiplexed fluorescence imaging and multimodal WSI. Acquiring an instant focal stack without z‐scanning may also enable fast 3‐dimensional dynamic tracking of various biological samples. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 1864063X
- Volume :
- 11
- Issue :
- 2
- Database :
- Complementary Index
- Journal :
- Journal of Biophotonics
- Publication Type :
- Academic Journal
- Accession number :
- 127900507
- Full Text :
- https://doi.org/10.1002/jbio.201700075