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Tracking HOCl concentrations across cellular organelles in real time using a super resolution microscopy probe.

Authors :
Ali, Firoj
Aute, Sunil
Sreedharan, Sreejesh
Anila, H. A.
Saeed, Hiwa K.
Smythe, Carl G.
Thomas, Jim A.
Das, Amitava
Source :
Chemical Communications; 2/21/2018, Vol. 54 Issue 15, p1849-1852, 4p
Publication Year :
2018

Abstract

BODIPY derivative, SF-1, exclusively shows a fluorescence ON response to HOCl and images endogenously generated HOCl in RAW 264.7 macrophages. Widefield and super resolution structured illumination microscopy images confirm localization in the Golgi complex and lysosomes, and hence specifically detects HOCl generated in these organelles. SF-1 is compatible with 3D-SIM imaging of individual cells. [ABSTRACT FROM AUTHOR]

Subjects

Subjects :
HYPOCHLORITES
ORGANELLES
HIGH resolution imaging
FLUORESCENCE microscopy
GLUTATHIONE

Details

Language :
English
ISSN :
13597345
Volume :
54
Issue :
15
Database :
Complementary Index
Journal :
Chemical Communications
Publication Type :
Academic Journal
Accession number :
128022456
Full Text :
https://doi.org/10.1039/c7cc09433g
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