Back to Search Start Over

Differential expression of antimicrobial peptides in corneal infection and regulation of antimicrobial peptides and reactive oxygen species by type III secretion system of Pseudomonas aeruginosa.

Differential expression of antimicrobial peptides in corneal infection and regulation of antimicrobial peptides and reactive oxygen species by type III secretion system of Pseudomonas aeruginosa.

Authors :
Sharma, Prerana
Guha, Sanjukta
Garg, Prashant
Roy, Sanhita
Source :
Pathogens & Disease; Feb2018, Vol. 76 Issue 1, p1-9, 9p
Publication Year :
2018

Abstract

Pseudomonas aeruginosa is an opportunistic pathogen and is the major cause of corneal infection worldwide that secret several virulent toxins through its type III secretion system (T3SS). In defense against pathogenic insults, epithelial cells and macrophages express antimicrobial peptides (AMPs) that are essential components of host immune response. In this study, we have determined the expression of several AMPs in patients with P. aeruginosa corneal infection. We also used an in vitro model of infection using human corneal epithelial cells and macrophages to determine the gene expression of AMPs and cellular response to wild-type and T3SS mutant P. aeruginosa. We found differential expression of several AMPs in patient samples and also found that P. aeruginosa repress AMP expression in both epithelial cells and macrophages by its T3SS in vitro. It dampens AMP expression by causing delay in NF-κB, p38 and ERK activation and inhibits reactive oxygen species generation in these cells by its T3SS. Our study show the profile of AMPs expressed during P. aeruginosa keratitis and suggest the pivotal role of the T3SS in epithelial cells and macrophages during P. aeruginosa infection. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
2049632X
Volume :
76
Issue :
1
Database :
Complementary Index
Journal :
Pathogens & Disease
Publication Type :
Academic Journal
Accession number :
128606585
Full Text :
https://doi.org/10.1093/femspd/fty001