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Lineage‐specific evolution of flavin‐containing monooxygenases involved in aliphatic glucosinolate side‐chain modification.
- Source :
- Journal of Systematics & Evolution; Mar2018, Vol. 56 Issue 2, p92-104, 13p
- Publication Year :
- 2018
-
Abstract
- Abstract: Glucosinolates, a class of specialized metabolites specific to the order Brassicales, have diverse bioactivities that are largely dependent on the structures of their side chains. Flavin‐containing monooxygenases (FMOs) encoded by the <italic>FMO<subscript>GS‐OX</subscript></italic> genes have been found to catalyze side‐chain modifications during the synthesis of methionine‐derived aliphatic glucosinolates. Seven <italic>FMO<subscript>GS‐OX</subscript></italic> genes have been identified in <italic>Arabidopsis</italic> Heynh., but the evolution of these genes in the Brassicaceae, a family including many economically important vegetables, is poorly understood. In this study, the phylogenetic and syntenic relationships of the <italic>FMO<subscript>GS‐OX</subscript></italic> genes belonging to 12 sequenced Brassicaceae species were analyzed. Our results showed that the <italic>FMO<subscript>GS‐OX</subscript></italic> genes included two tandem arrays, the <italic>FMO<subscript>GS‐OX2‐4</subscript></italic> group (group A) and the <italic>FMO<subscript>GS‐OX5‐7</subscript></italic> group (group B). The evolutionary histories of the <italic>FMO<subscript>GS‐OX</subscript></italic> groups A and B were similar across the Brassicaceae, but two lineage‐specific evolutionary routes developed after these two separate species lineages diverged from <italic>Aethionema arabicum</italic> (L.) Andrz. ex DC. In the lineage I route, <italic>FMO<subscript>GS‐OX</subscript></italic> gene copies tended to increase due to frequent tandem duplication events in most species and a whole genome triplication in <italic>Camelina sativa</italic> (L.) Crantz. In the lineage II route, gene copies decreased due to deletion events. Combining these results with those of previous studies, we speculated that the <italic>FMO<subscript>GS‐OX</subscript></italic> genes were derived from an ancestral gene with a broad expression distribution and a broad range of substrates, which then underwent subfunctionalization to generate progeny limited in either spatial expression or substrate structure. Furthermore, the absence of FMO<subscript>GS‐OX5</subscript> substrates in some <italic>FMO<subscript>GS‐OX5</subscript></italic>‐containing species may suggest neofunctionalization of these genes. [ABSTRACT FROM AUTHOR]
- Subjects :
- MONOOXYGENASES
GLUCOSINOLATES
BRASSICALES
FLAVINS
SUBSTITUENTS (Chemistry)
Subjects
Details
- Language :
- English
- ISSN :
- 16744918
- Volume :
- 56
- Issue :
- 2
- Database :
- Complementary Index
- Journal :
- Journal of Systematics & Evolution
- Publication Type :
- Academic Journal
- Accession number :
- 128680137
- Full Text :
- https://doi.org/10.1111/jse.12289