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Apolipoprotein M Protects Against Lipopolysaccharide-Induced Acute Lung Injury <italic>via</italic> Sphingosine-1-Phosphate Signaling.
- Source :
- Inflammation; Mar2018, Vol. 41 Issue 2, p643-653, 11p
- Publication Year :
- 2018
-
Abstract
- It had been demonstrated that apolipoprotein M (apoM) is an important carrier of sphingosine-1-phosphate (S1P) in blood, and the S1P has critical roles in the pathogenesis of sepsis-induced acute lung injury (ALI). In the present study, we investigated whether apoM has beneficial effects in a mouse model after lipopolysaccharide (LPS)-induced ALI. Forty-eight mice were divided into two groups: male C57BL/6 wild-type (apoM<superscript>+/+</superscript>) group (<italic>n</italic> = 24) and apoM gene-deficient (apoM<superscript>−/−</superscript>) group (n = 24) and then randomly subdivided into four subgroups (<italic>n</italic> = 6 each) according to different intraperitoneal (i.p.) injection: control group, W146 group, LPS group, and LPS + W146 group. Serum levels of interleukin-1 beta (IL-1β) and mRNA levels of IL-1β, interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α), lung histology, wet/dry weight ratio, and immunohistochemistry were measured at 3 h after the baseline and compared in each group. Our results clearly demonstrated that IL-1β mRNA levels and other inflammatory biomarkers were significantly increased in the lungs of LPS-induced ALI apoM<superscript>−/−</superscript> mice compared to those of the apoM<superscript>+/+</superscript> mice. Moreover, when apoM<superscript>+/+</superscript> mice were treated with W146, a S1P receptor (S1PR1) antagonist, these inflammatory biomarkers could be significantly upregulated by LPS-induced ALI. Therefore, it suggests that apoM-S1P-S1PR1 signaling might underlie the pathogenesis of ALI and apoM could have physiological benefits to alleviate LPS-induced ALI. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 03603997
- Volume :
- 41
- Issue :
- 2
- Database :
- Complementary Index
- Journal :
- Inflammation
- Publication Type :
- Academic Journal
- Accession number :
- 128746971
- Full Text :
- https://doi.org/10.1007/s10753-017-0719-x