Back to Search
Start Over
Severe Intestinal Inflammation in the Small Intestine of Mice Induced by Controllable Deletion of Claudin-7.
- Source :
- Digestive Diseases & Sciences; May2018, Vol. 63 Issue 5, p1200-1209, 10p
- Publication Year :
- 2018
-
Abstract
- <bold>Background: </bold>As a potential tumor suppressor gene, Claudin-7 (Cldn7), which is a component of tight junctions, may play an important role in colorectal cancer occurrence and development.<bold>Aims: </bold>To generate a knockout mouse model of inducible conditional Cldn7 in the intestine and analyze the phenotype of the mice after induction with tamoxifen.<bold>Methods: </bold>We constructed Cldn7-flox transgenic mice and crossed them with Villin-CreERT2 mice. The Cldn7 inducible conditional knockout mice appeared normal and were well developed at birth. We induced Cldn7 gene deletion by injecting different dosages of tamoxifen into the mice and then conducted a further phenotypic analysis.<bold>Results: </bold>After induction for 5 days in succession at a dose of 200 µl tamoxifen in sunflower oil at 10 mg/ml per mouse every time, the mice appeared dehydrated, had a lower temperature, and displayed inactivity or death. The results of hematoxylin-eosin staining showed that the intestines of the Cldn7 inducible conditional knockout mice had severe intestinal defects that included epithelial cell sloughing, necrosis, inflammation and hyperplasia. Owing to the death of ICKO mice, we adjusted the dose of tamoxifen to a dose of 100 µl in sunflower oil at 10 mg/ml per mouse (aged more than 8 weeks old) every 4 days. And we could induce atypical hyperplasia and adenoma in the intestine. Immunofluorescent staining indicated that the intestinal epithelial structure was destroyed. Electron microscopy experimental analysis indicated that the intercellular gap along the basolateral membrane of Cldn7 inducible conditional knockout mice in the intestine was increased and that contact between the cells and matrix was loosened.<bold>Conclusions: </bold>We generated a model of intestinal Cldn7 inducible conditional knockout mice. Intestinal Cldn7 deletion induced by tamoxifen initiated inflammation and hyperplasia in mice. [ABSTRACT FROM AUTHOR]
- Subjects :
- ENTERITIS
CLAUDINS
LABORATORY mice
TUMOR suppressor genes
TIGHT junctions
COLON cancer
HYPERPLASIA
ADENOMA
ANIMAL experimentation
BIOLOGICAL models
CELL membranes
COMPARATIVE studies
IMMUNOHISTOCHEMISTRY
SMALL intestine
INTESTINAL tumors
RESEARCH methodology
MEDICAL cooperation
MEMBRANE proteins
MICE
GENETIC mutation
RESEARCH
RESEARCH funding
SCANNING electron microscopy
TAMOXIFEN
WESTERN immunoblotting
PHENOTYPES
EVALUATION research
Subjects
Details
- Language :
- English
- ISSN :
- 01632116
- Volume :
- 63
- Issue :
- 5
- Database :
- Complementary Index
- Journal :
- Digestive Diseases & Sciences
- Publication Type :
- Academic Journal
- Accession number :
- 129021237
- Full Text :
- https://doi.org/10.1007/s10620-018-4973-z