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The Amino-Acid Sequence of Porcine Adenylate Kinase from Skeletal Muscle.
- Source :
- European Journal of Biochemistry; Mar74 Part 2, Vol. 43 Issue 1, p131-144, 14p
- Publication Year :
- 1974
-
Abstract
- 1. Adenylate kinase (ATP: AMP phosphotransferase) has been purified 490-fold from porcine muscle with a final yield of 60 mg/kg muscle. 2. The amino-acid composition is Asp<subscript>11</subscript> Asn<subscript>2</subscript>, Thr<subscript>14</subscript>, Ser<subscript>11</subscript>, Glu<subscript>19</subscript>,, Gln<subscript>6</subscript>, Pro<subscript>6</subscript>, Gly<subscript>19></subscript>, Ala<subscript>6</subscript>, Cyst<subscript>2</subscript>, Val<subscript>17</subscript>, Met<subscript>6</subscript>, Ile<subscript>9</subscript>, Leu<subscript>18</subscript>, Tyr<subscript>7</subscript>, Phe<subscript>5</subscript>, Lys<subscript>21</subscript>, His<subscript>2</subscript>, Arrg<subscript>11</subscript>. 3. The protein molecules a single polypeptide chain of 194 amino-acid residues with an acetylmethionine at the N-terminus and a lysine residue at the C-terminus. 4. Cyanogen bromide cleavage of carboxymethylated adenylate kinase yielded six fragments which were further degraded by using trypsin, chymotrypsin, thermolysin, subtilisin or α-protease. Sequence data on the resulting peptides are summarized in the present report, full details are given in a supplementary paper which has been deposited at CNRS from where copies can be obtained. 5. The primary structure of porcine adenylate kinase was given. [ABSTRACT FROM AUTHOR]
- Subjects :
- AMINO acid sequence
MUSCLES
SWINE
AMINO acids
PEPTIDES
PHOSPHOTRANSFERASES
Subjects
Details
- Language :
- English
- ISSN :
- 00142956
- Volume :
- 43
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- European Journal of Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 12903396
- Full Text :
- https://doi.org/10.1111/j.1432-1033.1974.tb03393.x