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Rosamine with pyronine-pyridinium skeleton: unique mitochondrial targetable structure for fluorescent probes.
- Source :
- Analyst; 4/21/2018, Vol. 143 Issue 8, p1813-1819, 7p
- Publication Year :
- 2018
-
Abstract
- Two rosamine-based probes (1a–b) with pyronine-pyridinium skeleton were designed and prepared. Probe 1a bearing boron ester unit was oxidized and eliminated upon addition of hydrogen peroxide (H<subscript>2</subscript>O<subscript>2</subscript>), and the emission spectrum exhibited OFF–ON response accompanied by 33-fold fluorescent enhancement. In contrast, the fluorescence intensity of probe 1b enhanced 58 times after the dinitrophenyl ether part within the probe was removed by nucleophilic substitution with hydrosulfide (H<subscript>2</subscript>S). The design concept was based on the d-PET process in pyronine-pyridinium structures, and free 4-pyridinyl-substituted pyronine dye showing strong fluorescence was released followed by elimination. Furthermore, as biocompatible molecules, probes 1a–b have been successfully applied for imaging in live HeLa and Ges-1 cells, and all of them can serve as mitochondrial targetable probes in red channel for detecting independent species. [ABSTRACT FROM AUTHOR]
- Subjects :
- PYRIDINIUM compounds
MITOCHONDRIA
REACTIVE oxygen species
Subjects
Details
- Language :
- English
- ISSN :
- 00032654
- Volume :
- 143
- Issue :
- 8
- Database :
- Complementary Index
- Journal :
- Analyst
- Publication Type :
- Academic Journal
- Accession number :
- 129101082
- Full Text :
- https://doi.org/10.1039/c7an02041d