The complete chloroplast genome of the green algae <italic>Hariotina reticulata</italic> (Scenedesmaceae, Sphaeropleales, Chlorophyta).

In this study, the chloroplast genome of <italic>Hariotina reticulata</italic> was fully sequenced and compared to other Sphaeropleales chloroplast genomes. It is 210,757 bp larger than most Sphaeropleales cpDNAs. It presents a traditional chloroplast structure, and contains 103 genes, including 68 protein-coding genes, six rRNA genes and 29 tRNA genes. The coding region constitutes of 43% of the whole cpDNA. Eighteen introns are found in 11 genes and six introns are unique for <italic>Hariotina</italic>. 11 open reading frames are identified among these introns. The synteny between <italic>Hariotina</italic> and <italic>Acutodesmus</italic> cpDNAs is in general identical, while within Sphaeropleales order, high variability in cpDNA architecture is indicated by general high DCJ distances. <italic>Ankyra judayi</italic> exhibits the greatest dissimilarity in gene synteny to the others and share some unique gene clusters with <italic>Treubaria triappendiculata</italic>. The phylogenomic analyses show that <italic>A. judayi</italic> is clustered with Treubariaceae species and sister to Chlorophyceae incertae sedis and other Sphaeropleales species. The monophyly of Sphaeropleales is rejected. [ABSTRACT FROM AUTHOR]