Back to Search
Start Over
research paper FLT-3 aberrations in acute promyelocytic leukaemia: clinicopathological associations and prognostic impact.
- Source :
- British Journal of Haematology; May2004, Vol. 125 Issue 4, p463-469, 7p
- Publication Year :
- 2004
-
Abstract
- FLT-3 aberrations that occur as an internal tandem duplication (ITD) or a mutation at the activation-loop position 835, D835, are common in acute promyelocytic leukaemia (APL). We investigated the clinicopathological associations and prognostic impact of FLT-3 aberrations in a cohort of APL patients. FLT-3 exons 11 and 12 were amplified by polymerase chain reaction (PCR), and the lTD was recognized as an increase in the size of the PCR product. FLT-3 exon 17 was amplified, and D835 mutation was identified by loss of an EcoRV site, followed by DNA sequencing. Of 82 patients studied, FLT-3 aberrations were detected in 35 cases (43%) at diagnosis (ITD: 16; D835 mutation: 18; lTD + D835 mutation: 1). FLT-3 ITD, but not D835 mutations, was significantly associated with higher presentation white blood cell count (WBC) and microgranular morphology. Early/induction deaths were related to male sex and high presentation WBC. There was a trend for FLT-3 lTD to be associated with non-remission (P = 0.06). For disease-free survival, high WBC was the only significant adverse factor. Male sex, high WBC and FLT-3 lTD were significant adverse factors for overall survival. These findings have important implications on the possible use of FLT-3 inhibitors in the treatment of APL. [ABSTRACT FROM AUTHOR]
- Subjects :
- GENETIC mutation
GENETICS
POLYMERASE chain reaction
BLOOD cells
DNA
MORPHOLOGY
Subjects
Details
- Language :
- English
- ISSN :
- 00071048
- Volume :
- 125
- Issue :
- 4
- Database :
- Complementary Index
- Journal :
- British Journal of Haematology
- Publication Type :
- Academic Journal
- Accession number :
- 12968574
- Full Text :
- https://doi.org/10.1111/j.1365-2141.2004.04935.x