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Phenolic profile, antioxidant and cytotoxic properties of polar extracts from leaves and flowers of Isatis tinctoria L. (Brassicaceae) growing in Sicily.

Authors :
Taviano, M. F.
Filocamo, A.
Ragusa, S.
Cacciola, F.
Dugo, P.
Mondello, L.
Paterniti Mastrazzo, G.
De Rose, R. F.
Celano, M.
Lombardo, G. E.
Melchini, A.
Miceli, N.
Source :
Plant Biosystems; Aug2018, Vol. 152 Issue 4, p795-803, 9p
Publication Year :
2018

Abstract

This study was designed to define and compare the antioxidant and cytotoxic properties of polar extracts obtained from basal leaves (It-BL), cauline leaves (It-CL) and flowers (It-F) of Isatis tinctoria L. growing wild around Acireale (Sicily, Italy). The phenolic profile was characterized by HPLC-PDA-ESI-MS analysis and the correlation between phenolic content and the observed biological effects was established. Further, LC/MS analysis showed that the extracts contain glucosinolates at very low concentrations. The antioxidant activity of the extracts was tested in vitro; It-F was the most effective in the DPPH test (IC<subscript>50</subscript> = 0.437 ± 0.003 mg/mL), whilst It-CL showed the best reducing power (1.546 ± 0.006 ASE/mL) and ferrous ions chelating activity (IC<subscript>50</subscript> = 0.564 ± 0.011 mg/mL). The extracts exhibited anti-proliferative effects against three different human thyroid carcinoma cell lines, and It-BL displayed the strongest activity; particularly, it markedly inhibited the growth of CAL-62 cells, causing nearly 85% reduction of viability at the highest tested dose. No cytotoxicity against Artemia salina was observed. The results of our investigations indicate that the polar extracts obtained from I. tinctoria are a potential source of antioxidant and anticancer compounds, which could be suitable for nutraceutical and therapeutic applications. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
11263504
Volume :
152
Issue :
4
Database :
Complementary Index
Journal :
Plant Biosystems
Publication Type :
Academic Journal
Accession number :
129815997
Full Text :
https://doi.org/10.1080/11263504.2017.1338629