Perfluorocarbons Prevent Lung Injury and Promote Development during Artificial Placenta Support in Extremely Premature Lambs.

<bold><italic>Background:</italic></bold> Extremely premature neonates suffer high morbidity and mortality. An artificial placenta (AP) using extracorporeal life support (ECLS) is a promising therapy. <bold><italic>Objectives:</italic></bold> We hypothesized that intratracheal perfluorocarbon (PFC) instillation during AP support would reduce lung injury and promote lung development relative to intratracheal amniotic fluid or crystalloid. <bold><italic>Methods:</italic></bold> Lambs at an estimated gestational age (EGA) 116–121 days (term 145 days) were placed on venovenous ECLS with jugular drainage and umbilical vein reinfusion and intubated. Airways were managed by the instillation of amniotic fluid and tracheal occlusion (TO; <italic>n</italic> = 4), or lactated Ringer’s (LR; <italic>n</italic> = 4) or perfluorodecalin (a PFC) without occlusion (<italic>n</italic> = 4). After 7 days, the animals were sacrificed. Early (EGA 116–121 days) and late (EGA 125–131 days) tissue control lambs were delivered and sacrificed. Lungs were formalin-inflated to 30 cm H₂O and sectioned for histology. Injury was scored by an unbiased pathologist. Slides were immunostained for PDGFR-α and α-actin; development was quantified by the area fraction of double-positive tips. Surfactant protein-C (SP-C) concentration in bronchoalveolar lavage fluid was quantified using ELISA. <bold><italic>Results:</italic></bold> Total injury scores were lower in PFC lungs (1.8 ± 1.7) than in TO (6.5 ± 2.1; <italic>p</italic> = 0.01) and LR lungs (5.5 ± 2.4; <italic>p</italic> = 0.01). The area fraction of double-positive alveolar tips appeared higher in PFC lungs than in TO lungs (0.18 ± 0.007 vs. 0.008 ± 0.004; <italic>p</italic> = 0.07). SP-C concentration was higher in PFC lungs than in TO lungs (37.9 ± 7.6 vs. 20.0 ± 5.4 pg/mL; <italic>p</italic> = 0.005), and both early (12.4 ± 1.7 g/mL; <italic>p</italic> = 0.007) and late tissue control lungs (15.1 ± 5.0 pg/mL; <italic>p</italic> = 0.0008). <bold><italic>Conclusion:</italic></bold> During AP support, intratracheal PFC prevents lung injury and promotes normal lung development better than crystalloid or amniotic fluid with TO. [ABSTRACT FROM AUTHOR]