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Biofilm Formation and Associated Genes in Listeria Monocytogenes.

Authors :
Warke, S. R.
Ingle, V. C.
Kurkure, N. V.
Tembhurne, P. A.
Prasad, Minakshi
Chaudhari, S. P.
Barbuddhe, S. B.
Source :
Indian Journal of Veterinary Sciences & Biotechnology; Jan-Apr2017, Vol. 12 Issue 3, p7-12, 6p
Publication Year :
2017

Abstract

Listeria monocytogenes, an opportunistic food borne pathogen can cause serious infections in immunocompromised individuals. L. monocytogenes is capable of producing biofilm on the surface of food processing lines and instruments.The biofilm transfers contamination to food products and impose risk to public health. In the present study biofilm producing ability of L. monocytogenes isolates were investigated phenotypically and genotypically by microtiter assay and multiplex PCR, respectively. Out of 38 L. monocytogenes isolates 14 were recovered from animal clinical cases, 12 bovine environment and 12 from milk samples. A total of 3 (21.42%) clinical, 2 (16.66%) environment and 3 (25%) milk samples respectively, revealed biofilm production in microtiter assay. Cumulative results showed that 23 (60.52%) out of 38 strains of L. monocytogenes were positive for luxS and flaA gene and 1 (2.63%) was positive only for the flaA gene. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
23940247
Volume :
12
Issue :
3
Database :
Complementary Index
Journal :
Indian Journal of Veterinary Sciences & Biotechnology
Publication Type :
Academic Journal
Accession number :
130340065
Full Text :
https://doi.org/10.21887/ijvsbt.v12i3.7079