Contour Primitives of Interest Extraction Method for Microscopic Images and Its Application on Pose Measurement.

This paper proposes a suite of methods to realize high precision pose measurement in 3-D Cartesian space based on a multicamera microscopic vision system. Since it is inefficient to develop a specific image algorithm for each kind of object and the imaging condition might be unsatisfactory, we propose a method of contour primitives of interest extraction, which allows flexible reconfiguration for novel object image and owns robustness under different imaging conditions. The object is detected in grayscale image based on a template of contour primitives. Edges are extracted according to derivatives along the normal vectors of these contour primitives. The positions and directional derivatives of these edges are used for feature extraction and autofocus, respectively. The point features and line features extracted from multiview images are utilized to measure 3-D vectors and orientations, respectively, based on image Jacobian matrices. Cameras’ linear motions are considered in the imaging model, so that the measurement range is expanded beyond the limitation of microscopes’ shallow depths of field. The affine epipolar constraint and focused planes intersection constraint between cameras are applied to improve the real time performances of image feature extraction and multicamera autofocus, respectively. A series of experiments are conducted to verify the effectiveness of the proposed methods. The root mean square errors of pose measurement are evaluated as 3 ${ {\mu } }\text{m}$ in position and 0.05° in orientation, while the measurement range is about 5000 $ {\mu }\text{m}$ in position and 20° in orientation. [ABSTRACT FROM AUTHOR]