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Repurposing Ionophores as novel antimicrobial agents for the treatment of bovine mastitis caused by Gram‐positive pathogens.

Authors :
Hickey, Elizabeth E.
Wong, Hui San
Khazandi, Manouchehr
Ogunniyi, Abiodun D.
Petrovski, Kiro R.
Garg, Sanjay
Page, Stephen W.
O'Handley, Ryan
Trott, Darren J.
Source :
Journal of Veterinary Pharmacology & Therapeutics; Oct2018, Vol. 41 Issue 5, p746-754, 9p
Publication Year :
2018

Abstract

Abstract: Increasing reports of multidrug‐resistant bacterial infections in animals has created a need for novel antimicrobial agents that do not promote cross‐resistance to critically important antimicrobial classes used in human medicine. In response to the recent emergence of antimicrobial resistance in several bovine mastitis pathogens, in vitro antimicrobial susceptibility was determined for four polyether ionophores (lasalocid, monensin, narasin and salinomycin) against Staphylococcus spp. and Streptococcus spp. isolated from clinical cases. In addition, erythrocyte haemolysis and WST‐1 cell proliferation assays were used to assess in vitro mammalian cell cytotoxicity and biofilm susceptibility testing was performed using the minimum biofilm eradication concentration (MBEC™) biofilm assay. Lasalocid, monensin, narasin and salinomycin exhibited bacteriostatic antimicrobial activity against all pathogens tested, including methicillin‐resistant staphylococci, with MIC<subscript>90</subscript> values <16 μg/ml. Narasin and monensin displayed the least toxicity against mammalian cell lines and all compounds significantly reduced viable cell numbers in a Staphylococcus aureus biofilm. Based on in vitro characterization, all four ionophores offer potentially novel treatments against bovine mastitis but in vivo studies will be essential to determine whether acceptable safety and efficacy is present following intramammary administration. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
01407783
Volume :
41
Issue :
5
Database :
Complementary Index
Journal :
Journal of Veterinary Pharmacology & Therapeutics
Publication Type :
Academic Journal
Accession number :
131412023
Full Text :
https://doi.org/10.1111/jvp.12674