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Draft Genome and Description of Eisenbergiella massiliensis Strain AT11T: A New Species Isolated from Human Feces After Bariatric Surgery.
- Source :
- Current Microbiology; Oct2018, Vol. 75 Issue 10, p1274-1281, 8p
- Publication Year :
- 2018
-
Abstract
- A novel strain of a Gram-stain negative, non-motile, non-spore forming rod-shaped, obligate anaerobic bacterium, designated AT11<superscript>T</superscript>, was isolated from a stool sample of a morbidly obese woman living in Marseille, France. This bacterium was characterized using biochemical, chemotaxonomic, and phylogenetic methods. The 16S rRNA gene sequence analysis showed that strain AT11<superscript>T</superscript> had a 97.8% nucleotide sequence similarity with Eisenbergiella tayi strain B086562<superscript>T</superscript>, the closest species with standing in nomenclature. The major cellular fatty acids of the novel isolate were C<subscript>16:0</subscript> followed by saturated or unsaturated C<subscript>18</subscript> fatty acids (C<subscript>18:1</subscript>n9, C<subscript>18:1</subscript>n5 and C<subscript>18:0)</subscript>. The draft genome of strain AT11<superscript>T</superscript> is 7,114,554 bp long with 48% G+C content. 6176 genes were predicted, including 6114 protein-coding genes and 62 were RNAs (with 2 5S rRNA genes, two 16S rRNA genes, two 23S rRNA genes, and 56 tRNA genes). The digital DNA-DNA hybridization (dDDH) relatedness between the new isolate and E. tayi strain B086562<superscript>T</superscript> was 23.1% ± 2.2. Based on the phenotypic, chemotaxonomic, genomic, and phylogenetic characteristics, Eisenbergiella massiliensis sp. nov., is proposed. The type strain is AT11<superscript>T</superscript> (= DSM 100838<superscript>T</superscript> = CSUR P2478<superscript>T</superscript>). [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 03438651
- Volume :
- 75
- Issue :
- 10
- Database :
- Complementary Index
- Journal :
- Current Microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 131455575
- Full Text :
- https://doi.org/10.1007/s00284-018-1520-2