Back to Search
Start Over
p40 molecule regulates NK cell activation mediated by NK receptors for HLA class I antigens and TCR-mediated triggering of T lymphocytes.
- Source :
- International Immunology; Sep1997, Vol. 9 Issue 9, p1271-1279, 9p, 3 Charts, 5 Graphs
- Publication Year :
- 1997
-
Abstract
- p40 was previously described as a regulatory molecule capable of inhibiting both the natural and the CD16-mediated cytotoxicity of NK cells. In this study, we analyze the effect of p40 molecule engagement on the NK cell triggering induced by activating HLA class I-specific NK receptors (NKR) or on TCRαβ-mediated T cell activation. CD3<superscript>–</superscript>CD16<superscript>+</superscript> NK cell clones expressing activating NKR (either CD94 or p50) were analyzed in a redirected killing assay using P815 target cells and appropriate mAb. A strong target cell lysis was detected in the presence of anti-NKR or anti-CD16 mAb alone. Addition of anti-p40 mAb resulted in a strong inhibition of both anti-NKR or anti-CD16 mAb-induced cytolysis. mAb specific for either CD45 or lymphocyte function associated antigen-1 did not exert any inhibitory effect in the same experimental system. Free intracellular calcium ([Ca<superscript>2</superscript>+]i) increase induced by mAb cross-linking of activating CD94 or p50 was inhibited by simultaneous engagement of p40 molecules, but not of other NK surface molecules including CD44 and CD56. In addition, cross-linking of p40 molecules strongly inhibited the CD94-induced tumor necrosis factor-α and IFN-γ production. Analysis of TCR αβ or γδ T cell clones revealed that the engagement of p40 molecules, using specific mAb, induced some degree of inhibition only on anti-V<subscript>β</subscript> (but not anti-V<subscript>δ</subscript> or anti-CD3) mAb-induced cytotoxicity. On the other hand, the p40 molecule engagement prevented T cell proliferation induced by either anti-V<subscript>β</subscript>8 or anti-V<subscript>δ</subscript>2 mAb. A similar inhibitory effect was found on the IL-2-induced NK cell proliferation. Taken together, our present findings suggest that p40 may play a role in the regulation of NK and T lymphocyte activation and proliferation. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09538178
- Volume :
- 9
- Issue :
- 9
- Database :
- Complementary Index
- Journal :
- International Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 131633507
- Full Text :
- https://doi.org/10.1093/intimm/9.9.1271