Back to Search
Start Over
Clinical evaluation of a panel of multiplex quantitative real-time reverse transcription polymerase chain reaction assays for the detection of 16 respiratory viruses associated with community-acquired pneumonia.
- Source :
- Archives of Virology; Oct2018, Vol. 163 Issue 10, p2855-2860, 6p
- Publication Year :
- 2018
-
Abstract
- We developed a panel of multiplex quantitative real-time reverse transcription polymerase chain reaction (mqRT-PCR) assay consisting of seven internally controlled qRT-PCR assays to detect 16 different respiratory viruses. We compared the new mqRT-PCR with a previously reported two-tube mRT-PCR assay using 363 clinical sputum specimens. The mqRT-PCR assay performed comparably with the two-tube assay for most viruses, offering the advantages of quantitative analysis, easier performance, lower susceptibility to contamination, and shorter turnaround time in laboratories equipped with conventional real-time PCR instrumentation, and it could therefore be a valuable tool for routine surveillance of respiratory virus infections in China. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 03048608
- Volume :
- 163
- Issue :
- 10
- Database :
- Complementary Index
- Journal :
- Archives of Virology
- Publication Type :
- Academic Journal
- Accession number :
- 131705209
- Full Text :
- https://doi.org/10.1007/s00705-018-3921-8