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The Significance of the Protein Carrier in the Stimulation of DNA Synthesis by Hapten-Protein Conjugates in the Secondary Response.
- Source :
- Immunology; Jan64, Vol. 7 Issue 1, p54-64, 11p
- Publication Year :
- 1964
-
Abstract
- Rabbits were immunized with 2,4-dinitrophenyl (DNP)-protein conjugates. Spleen cell suspensions were prepared and incubated in the presence of various DNP protein conjugates, the proteins alone, and DNP-lysine. The antigen dependent stimulation of DNA synthesis was used as a measure of the antigenic ‘activity’ of the DNP preparations. It was found that the cells were strongly stimulated by the DNP-protein conjugates used for immunization, and weakly stimulated by the protein alone. Highly substituted DNP protein conjugates were markedly more effective than lightly substituted conjugates. DNP-conjugates with proteins other than the one used during immunization were inactive. DNP-lysine alone was inactive but inhibited stimulation by the DNP protein conjugate used for immunization. The significance of these findings is discussed. [ABSTRACT FROM AUTHOR]
- Subjects :
- DNA synthesis
IMMUNITY
LABORATORY animals
DINITROBENZENES
ANTIGENS
PROTEINS
Subjects
Details
- Language :
- English
- ISSN :
- 00192805
- Volume :
- 7
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 13272372