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A Quantitative Study of Antigen-Antibody Combination during Disk Electrophoresis in Acrylamide Gel using Iodine-131 Labelled Human Growth Hormone.
- Source :
- Immunology; May64, Vol. 7 Issue 3, p307-315, 9p
- Publication Year :
- 1964
-
Abstract
- An adaptation of the technique of disk electrophoresis is described. It allows combination of antigen and antibody, as well as the separation of the free antigen from the complex, in a single electrophoretic step. An Iodine-131 labelled human growth hormone : antihuman growth hormone system was used to demonstrate this combination which was expressed as per cent radioactivity bound to immune γ-globulin. Dilution of antiserum gave reproducible titration curves when run at three different temperatures: 10°, 20°, 35°. The curves obtained at 35° showed increased binding. The reduction of bound radioactivity on addition of unlabelled growth hormone to the sample was a linear function of the logarithm of unlabelled growth hormone. The standard curves obtained are suitable for a highly sensitive assay of human growth hormone. [ABSTRACT FROM AUTHOR]
- Subjects :
- IMMUNOLOGY
IMMUNE complexes
ELECTROPHORESIS
SOMATOTROPIN
ANTIGENS
IMMUNOGLOBULINS
Subjects
Details
- Language :
- English
- ISSN :
- 00192805
- Volume :
- 7
- Issue :
- 3
- Database :
- Complementary Index
- Journal :
- Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 13274458