SpoVID functions as a non‐competitive hub that connects the modules for assembly of the inner and outer spore coat layers in Bacillus subtilis.

Summary: During sporulation in Bacillus subtilis, a group of mother cell‐specific proteins guides the assembly of the coat, a multiprotein structure that protects the spore and influences many of its environmental interactions. SafA and CotE behave as party hubs, governing assembly of the inner and outer coat layers. Targeting of coat proteins to the developing spore is followed by encasement. Encasement by SafA and CotE requires E, a region of 11 amino acids in the encasement protein SpoVID, with which CotE interacts directly. Here, we identified two single alanine substitutions in E that prevent binding of SafA, but not of CotE, to SpoVID, and block encasement. The substitutions result in the accumulation of SafA, CotE and their dependent proteins at the mother cell proximal spore pole, phenocopying a spoVID null mutant and suggesting that mislocalized SafA acts as an attractor for the rest of the coat. The requirement for E in SafA binding is bypassed by a peptide with the sequence of E provided in trans. We suggest that E allows binding of SafA to a second region in SpoVID, enabling CotE to interact with E and SpoVID to function as a non‐competitive hub during spore encasement. Assembly of the inner and outer coat of Bacillus subtilis spores is governed by SafA and CotE, which recruit the inner and outer coat proteins to the developing spore. Encasement of the spore by the coat layers require binding of SafA and CotE to a region of 12 residues in the encasement protein SpoVID. Despite the proximity and partial overlap of the binding sites in SpoVID, the inner and outer coat modules are largely independent. [ABSTRACT FROM AUTHOR]