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Screening of Sesame Cultivars with Scant Albumins by an Immunological Approach.
- Source :
- Food Biotechnology; 2018, Vol. 32 Issue 4, p273-285, 13p, 3 Color Photographs, 1 Chart, 3 Graphs
- Publication Year :
- 2018
-
Abstract
- In this study a detection method for 2S albumin (14-kDa albumin) among the sesame allergens was developed. Polyclonal antibodies against 14-kDa albumin from sesame seeds (anti 14-kDa albumin antibody) were prepared. The anti 14-kDa albumin antibody absorbed with acetone powder from soybean seeds specifically reacted with sesame albumin, and did not react with albumins from soybean, corn, Arabidopsis. The absorbed anti 14-kDa albumin antibody was used as a probe for screening sesame varieties with scant albumins by indirect enzyme-immunosorbent assay (ELISA). We isolated accession number (Acc. No.) 526 with one-third of albumin amounts compared to standard variety Acc. No. 800. This method will be useful in breeding sesame cultivars with low sesame allergens [ABSTRACT FROM AUTHOR]
- Subjects :
- ALBUMINS
SESAME
ALLERGENS
IMMUNOGLOBULINS
SOYBEAN
ENZYME-linked immunosorbent assay
Subjects
Details
- Language :
- English
- ISSN :
- 08905436
- Volume :
- 32
- Issue :
- 4
- Database :
- Complementary Index
- Journal :
- Food Biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 133291176
- Full Text :
- https://doi.org/10.1080/08905436.2018.1519445