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Molecular characterization of plasmid-encoded Tripoli MBL 1 (TMB-1) in Enterobacteriaceae.
- Source :
- Journal of Antimicrobial Chemotherapy (JAC); Jan2019, Vol. 74 Issue 1, p42-47, 6p
- Publication Year :
- 2019
-
Abstract
- <bold>Objectives: </bold>Available commercial tools (molecular methods or immunochromatographic assays) usually allow the detection of the five most prevalent carbapenemases (KPC, NDM, VIM, IMP and OXA-48-like), but miss minor carbapenemases. Here, we characterize two enterobacterial isolates with reduced susceptibility to carbapenems and negative for the most commonly encountered carbapenemase genes.<bold>Methods: </bold>Enterobacter hormaechei and Citrobacter freundii isolates were recovered from a bile sample and rectal screening, respectively. Both isolates were investigated by WGS. Resistance genes were detected using ResFinder. The blaTMB-1-harbouring plasmid was reconstructed using CLC genomic workbench 10.0 and was annotated using the RAST tool. Transfer frequency was determined by conjugation experiments using the laboratory strain Escherichia coli J53.<bold>Results: </bold>The two isolates were resistant to broad-spectrum cephalosporins and carbapenems. WGS revealed the presence of blaTMB-1, which has previously only been described in non-fermenters. blaTMB-1 was located within an ISKpn19-based composite class 1 transposon. Comparative genomics revealed that this structure was carried on a conjugative IncN-type plasmid within an integration hotspot. Conjugation experiments revealed high transfer frequencies of ∼1 × 10-3.<bold>Conclusions: </bold>To the best of our knowledge, this study corresponds to the first report of Tripoli MBL 1-producing Enterobacteriaceae. Despite always being described as likely to be chromosomally located in non-fermenters, the blaTMB-1 gene is now found to be carried by a conjugative plasmid among Enterobacteriaceae, raising concern about the possible dissemination of this carbapenemase. The blaTMB-1 gene should now be suspected when PCRs targeting the main carbapenemases remain negative. [ABSTRACT FROM AUTHOR]
- Subjects :
- CARBAPENEMS
GENE expression
DRUG resistance in bacteria
PATHOGENIC microorganisms
KLEBSIELLA pneumoniae
BILE
CEPHALOSPORINS
COMPARATIVE studies
DRUG resistance in microorganisms
ESCHERICHIA coli
GENES
GENETICS
GRAM-negative bacteria
HYDROLASES
RESEARCH methodology
MEDICAL cooperation
RECTUM
RESEARCH
EVALUATION research
CITROBACTER
ENTEROBACTERIACEAE diseases
PHARMACODYNAMICS
Subjects
Details
- Language :
- English
- ISSN :
- 03057453
- Volume :
- 74
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Journal of Antimicrobial Chemotherapy (JAC)
- Publication Type :
- Academic Journal
- Accession number :
- 133582959
- Full Text :
- https://doi.org/10.1093/jac/dky372