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Mechanisms of lymphocyte adhesion to endothelial cells: studies using a LFA-1-deficient cell line.
- Source :
- Immunology; Jan1989, Vol. 66 Issue 1, p111-116, 6p
- Publication Year :
- 1989
-
Abstract
- In order to investigate the role of lymphocyte function-associated antigen 1 (LFA-1) in lymphocyte adhesion to endothelial cells (EC), we have studied the adhesion of a LFA-1-deficient lymphoblastoid cell line, ICH-KM, which has < 10% of the cell surface LFA-1 expressed on a normal lymphoblastoid cell line, ICH-BJ. The adhesion of ICH-KM cells to unstimulated EC was 49.9 ± 8-6% (mean ± SD) that of ICH-BJ cells. Moreover, phorbol ester-stimulated ICH-KM cells showed a considerably weaker increase in adhesion to unstimulated EC compared with ICH-BJ cells (mean ± SD increase in percentage adhesion, 3.8±2-3 compared with 18.5±8.0; P<0.025). In contrast, there was no significant difference between the enhanced adhesion of ICH-KM cells and ICH-BJ cells to interleukin-1 (IL-1)-stimulated EC. Thus ICH-KM cells showed a 22.7 ± 11.0 (mean ± SD) increase in percentage adhesion to IL-1-stimulated EC compared with the 24.8 ± 8-5 increase in percentage adhesion of ICH-BJ cells. Anti-LFA-1 monoclonal antibodies had no effect on the enhanced adhesion of ICH-KM and ICH-BJ cells to IL-1-stimulated EC but abolished the differences in adhesion between the two cell lines. The study therefore indicates that although a major part of unstimulated and phorbol ester-stimulated lymphocyte-EC adhesion is dependent upon LFA-1, the enhanced adhesion due to stimulation of EC with IL-1 is not dependent upon this molecule. The data therefore supports the existence of cytokine-inducible LFA-1-independent adhesion molecules for lymphocytes on EC. [ABSTRACT FROM AUTHOR]
- Subjects :
- LYMPHOCYTES
LYMPHOBLASTOID cell lines
CELLS
ANTIGENS
PHORBOLS
MONOCLONAL antibodies
Subjects
Details
- Language :
- English
- ISSN :
- 00192805
- Volume :
- 66
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 13363371