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The immune response to oxidized ferredoxin II. CROSS REACTIVITY OF CELLS AND ANTISERA TO MODIFIED FERREDOXINS AND THE NATURE OF THE CELLS RESPONDING IN VITRO.
- Source :
- Immunology; Sep76, Vol. 31 Issue 3, p379-388, 10p
- Publication Year :
- 1976
-
Abstract
- The cross reactivity of sera from rabbits sensitized to performic acid oxidized ferredoxin (O-Fd) and of spleen cells from mice sensitized to O-Fd was analysed using several chemically modified forms of ferredoxin in the complement fixation test and the in vitro lymphocyte stimulation assay. Only O-Fd and native ferredoxin (native-Fd) gave positive responses in both assays. Dinitrophenylated-O-Fd (DNP-O-Fd) and acid precipitated ferredoxin (TCA-Fd) were able to fix complement (C') but did not simulate DNA synthesis in vitro. Ferredoxin alkylated with N-ethylmaleimide (NEM-Fd) induced cell transformation but fixed C' poorly. Carboxymethylated ferredoxin (CM-Fd) was unable to stimulate DNA synthesis and was marginally able to fix C'. Methylated-O-Fd (meth-O-Fd) was not recognized in either assay. The various ferredoxin preparations were tested for their ability to sensitize mice for use in the in vitro lymphocyte stimulation assay. Only O-Fd, NEM-Fd and native-Fd were capable of sensitizing lymphocytes for a proliferative response in vitro to the test antigens. This correlates with the observation that only these antigens were able to induce DNA synthesis in O-Fd-sensitized lymphocytes. The nature of the cells responding in vitro was examined by treating the cells with rabbit antimouse irranunoglobulin and C' or rabbit anti-mouse brain associated θ and C'. The 24-hr response was found to be sensitive to both sera while the 120-hr response was sensitive only to the anti-θ sera. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00192805
- Volume :
- 31
- Issue :
- 3
- Database :
- Complementary Index
- Journal :
- Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 13372736