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Secondary cytotoxic cell response to lymphocytic choriomeningitis virus II. NATURE AND SPECIFICITY OF EFFECTOR CELLS.
- Source :
- Immunology; Aug76, Vol. 31 Issue 2, p181-186, 6p
- Publication Year :
- 1976
-
Abstract
- The method described in the previous paper was used to induce secondary responses in spleen cells from CBA/H mice, pre-primed with lymphocytic choriomeningitis (LCM) virus by culturing them with LCM-infected peritoneal cells. The cytolytic effector cells thus generated have been characterized. Effector cells were sensitive to treatment with anti-θ ascitic fluid and complement. Separation procedures based on rosetting of certain categories of lymphocytes with sheep red cells through an isopaque-Ficoll gradient indicated that effector cells lacked surface immunoglobulin and generally did not bear Fc receptors. Cytolytic activity was restricted by the H-2 gene complex. Killing had single-hit characteristics. All these results suggested that the cells from memory cultures mediating cytolysis were T cells. There was evidence for two T cell subsets, a major subpopulation directed against antigens on infected targets and a minor one directed against antigens on uninfected, H-2-compatible targets. Specificity was present at the infected cell: memory responder and killer:target levels between LCM virus (an arenavirus) and ectromelia virus (a poxvirus). [ABSTRACT FROM AUTHOR]
- Subjects :
- IMMUNE response
IMMUNOLOGY
SPLEEN
LYMPHOID tissue
FC receptors
CELL receptors
Subjects
Details
- Language :
- English
- ISSN :
- 00192805
- Volume :
- 31
- Issue :
- 2
- Database :
- Complementary Index
- Journal :
- Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 13373185