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Studies on the Distribution of Macrophages Derived from Rat Bone Marrow Cells in Xenogeneic Radiation Chimaeras.
- Source :
- Immunology; Apr72, Vol. 22 Issue 4, p549-556, 8p
- Publication Year :
- 1972
-
Abstract
- Lethally-irradiated mice were injected with rat bone marrow cells and examined subsequently for the presence of rat macrophages, using an indirect fluorescent antibody test in conjunction with a fluorochrome-labelled phagocytic cell marker. Donor bone marrow cells were fractionated by (1) adherence, (2) magnetic removal of phagocytic cells containing ingested carbonyl iron and (3) treatment with lymphocyte-absorbed anti-rat macrophage serum either in vitro or in vivo. Extensive rat cell proliferation occurred in the resulting xenogeneic chimaeras by or after Day 4 and increased during the next week. The precursor of both the free and fixed macrophages was contained in the non-adherent, non-phagocytic population of bone marrow cells. The anti-macrophage serum was not selective and abolished the capability of bone marrow cells to proliferate. [ABSTRACT FROM AUTHOR]
- Subjects :
- MACROPHAGES
BONE marrow cells
LABORATORY mice
LYMPHOCYTES
PHAGOCYTOSIS
IMMUNOLOGY
Subjects
Details
- Language :
- English
- ISSN :
- 00192805
- Volume :
- 22
- Issue :
- 4
- Database :
- Complementary Index
- Journal :
- Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 13377334