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Non-specific factor enhancement of human <em>in vitro</em> antigen-dependent antibody synthesis: role of B cell activation and T cell help.
- Source :
- Immunology; Apr84, Vol. 51 Issue 4, p783-791, 9p
- Publication Year :
- 1984
-
Abstract
- Lectin-free supernatants obtained from PWM-stimulated lymphocytes, enable B cells to proliferate and secrete immunoglobulin. Both functions arc augmented by the addition of irradiated T cells. In the presence of antigen, these supernatants also enhance specific anti-tetanus toxoid antibody production. The components of the supernatant responsible for these activities have a molecular weight between 30,000 and 60,000, and have the characteristics of non-specific factors: they are genetically unrestricted, and do not bind to either antigen or anti-DR affinity columns. There is no evidence that the partial T dependency of these factors is an indication that their target is a T cell. Instead, T cells appear necessary to move the B cell into a state of activation in which it becomes responsive to the factor. Alternative activation signals such as Staph. A. Cowan can substitute for T cell help in the proliferative response, but not for immunoglobulin or antibody synthesis. The implications of these results for the approaches used to detect and classify B cell growth factors are discussed. [ABSTRACT FROM AUTHOR]
- Subjects :
- LYMPHOCYTES
T cells
IMMUNOGLOBULINS
ANTIGENS
B cells
GROWTH factors
Subjects
Details
- Language :
- English
- ISSN :
- 00192805
- Volume :
- 51
- Issue :
- 4
- Database :
- Complementary Index
- Journal :
- Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 13385102