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Overexpression of Fbxo6 inactivates spindle checkpoint by interacting with Mad2 and BubR1.
- Source :
- Cell Cycle; 2018, Vol. 17 Issue 24, p2779-2789, 11p
- Publication Year :
- 2018
-
Abstract
- The spindle assembly checkpoint prevents chromosome mis-segregation during mitosis by delaying sister chromatid separation. Several F-box protein members play critical roles in maintaining genome stability and regulating cell cycle progress via ubiquitin-mediated protein degradation. Here, we showed that Fbxo6 critically regulated spindle checkpoint and chromosome segregation. Fbxo6 was phosphorylated during mitosis. Overexpression of Fbxo6 lead to faster exit from nocodazole-induced mitosis arrest through premature sister chromatid separation. Moreover, we found substantially more binuclear and multilobed nuclei cells accompanied with impaired cell viability in Fbxo6-overexpressed HeLa cells. Mechanistically, Fbxo6 interacted with spindle checkpoint proteins including Mad2 and BubR1 leading to the premature exit from mitosis. Overall, we revealed a novel role of Fbxo6 in regulating spindle checkpoint, which may shed light on the regulation of genome instability of cancer cells. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 15384101
- Volume :
- 17
- Issue :
- 24
- Database :
- Complementary Index
- Journal :
- Cell Cycle
- Publication Type :
- Academic Journal
- Accession number :
- 133958982
- Full Text :
- https://doi.org/10.1080/15384101.2018.1557488