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Changes occurring on the surface of mouse T cells during concanavalin A-induced lymphoblastic transformation.
- Source :
- Immunology; Dec79, Vol. 38 Issue 4, p827-834, 8p
- Publication Year :
- 1979
-
Abstract
- Thy-1 antigen and GPCA-the mouse T-cell surface component responsible for activation of guinea-pig complement (C)-were readily demonstrated on unstimulated but not on Con A-stimulated thymus or spleen cells when (guinea-pig) C-dependent cytotoxic reactions were used. Mitogenic stimulation was a necessary condition for these changes to occur since mere incubation of thymocytes in the absence of Con A failed to alter the sensitivity of these cells to treatment with either anti-Thy-l.2 antibodies plus C (devoid of non-specific cytotoxicity) or untreated guinea-pig serum (GPS) alone. Both Thy-l.2 and GPCA were, however, readily detectable on the Con A blasts when tested for by indirect immunofluorescence and immune adherence, respectively, i.e. by using tests which do not involve C-effected lysis. That Thy-1 was indeed expressed on the T-cell blasts was further indicared by their capacity to absorb anti-Thy-l.2 activity from specific antiserum. Con A, whether bound to the cells or added in excess to the reaction mixture, did not interfere with C-mediated lysis and was ruled out as a possible C inactivator. Pre-treatment of the lymphoblasts with neuraminidase rendered these cells sensitive to lysis by either anti-Thy-l.2 plus C or GPS. These results, highlighting altered reactivity of mouse T lymphoblasts with guinea-pig C, indicated the occurrence of surface changes during mitogen-induced blastogenesis and suggested a role for sialic acid residues in interference with C-dependent cylotoxicity. [ABSTRACT FROM AUTHOR]
- Subjects :
- T cells
LYMPHOCYTES
LYMPHOBLASTOID cell lines
IMMUNOGLOBULINS
GLOBULINS
PLASMA cells
Subjects
Details
- Language :
- English
- ISSN :
- 00192805
- Volume :
- 38
- Issue :
- 4
- Database :
- Complementary Index
- Journal :
- Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 13396012