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<em>In vivo</em> modulation of thymus-derived lymphocytes with monoclonal antibodies in mice I. EFFECT OF ANTI-THY-1 ANTIBODY ON THE TISSUE DISTRIBUTION OF LYMPHOCYTES.
- Source :
- Immunology; Dec83, Vol. 50 Issue 4, p537-546, 10p
- Publication Year :
- 1983
-
Abstract
- A procedure is described for the in vivo removal of all detectable T lymphocytes from spleen and lymph nodes in mice. A single intraperitoneal injection of monoclonal anti-Thy-T antibody into mice leads to rapid depletion of functional I cells from peripheral lymphoid organs, but not thymus. The extent of T-celI depletion is dependent on the cytotoxic titre of the anti-Thy-I antibody used. Antibody with a median cytotoxic titre greater than 10<superscript>6</superscript> causes the complete removal of cells bearing Thy-I, Lyt-I and Lyt-2 surface antigens from peripheral lymphoid populations in 3 days. Eight days after treatment Thy-I <superscript>+</superscript>, Lyt-I <superscript>+</superscript> and Lyt-2<superscript>+</superscript> cells begin to reappear in these organs. Splenic B cells, assayed by the expression of surface immunoglobulin (sig) and by mitogenic responsiveness to bacterial lipopolysaccharide (LPS), are not affected by this treatment. The monoclonal anti-Thy-i antibody does not appear to penetrate thymus tissue and bind to thymocytes. Anti-Thy-I antibody, but not F(ab')<subscript>2</subscript>, is required for in-vivo T-cell depletion. These findings indicate that anti-Thy-I antibody causes the removal of Thy-i <superscript>+</superscript> cells from peripheral lymphoid tissue, and as the circulating levels of anti-Thy-I antibody decrease, cells from the thymus repopulate the thymus-dependent areas of the depleted lymphoid organs. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00192805
- Volume :
- 50
- Issue :
- 4
- Database :
- Complementary Index
- Journal :
- Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 13399666