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Enhancement of immunogenicity by incorporation of lipid A into liposomal model membranes and its application to membrane-associated antigens.
- Source :
- Immunology; Dec83, Vol. 50 Issue 4, p605-612, 8p
- Publication Year :
- 1983
-
Abstract
- Incorporation of lipid A into liposomes markedly enhances the plaque-forming cell (PFC responses against model membranes that are sensitized with synthetic amphipathic antigens 2,4-dinitrophenyl-6-N-aminocaproyldipalmytoylphosphatidylethanolamine (DNP-Cap-DPPE) and fluorescein thiocarbamyldipalmytoylphosphatidylethanolamine (FI-DPPE). The enhancing effect was pronounced only when lipid A and haptenic determinants were presented on the same liposome. Lipid A was incorporated into heterologous erythrocytes and syngeneic tumour cells. Significant and selective increase of in-vitro PFC responses was observed against heterologous erythrocytes, and immunization of C578L/6 mice with lipid A treated X-irradiated tumour cells (EL-4) markedly prolonged the survival of mice after challenge with homologous viable tumour cells. These data suggest that passively incorporated lipid A will be an effective method for selectively augmenting antibody formation against membrane-associated antigens. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00192805
- Volume :
- 50
- Issue :
- 4
- Database :
- Complementary Index
- Journal :
- Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 13400324