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Primary T-cell responses to minor alloantigens I. CHARACTERIZATION OF CYTOTOXIC EFFECTOR CELLS GENERATED FROM REGIONAL LYMPH NODES AFTER IMMUNIZATION IN THE FOOTPAD.
- Source :
- Immunology; Sep83, Vol. 50 Issue 1, p121-129, 9p
- Publication Year :
- 1983
-
Abstract
- A system is described which involves an in-vivo and an in-vitro step for the generation of strong primary cytotoxic T lymphocyte (CTL) responses to multiple minor alloantigens. The protocol consists of immunizing mice in the footpad with irradiated, minor alloantigen-different spleen cells. Four days later, cells from the lymph nodes draining the site of injection are cultured for 3 days in the absence of antigen to allow the development of cytotoxic activity. The analysis of the effector phase of the response indicates that the killer cells are H-2 restricted cytotoxic cells specific for minor alloantigens. The in-vitro phase of these cytotoxic responses is I cell-dependent and the responder cells proliferate strongly during the culture period. Both cytotoxic and proliferative responses are abolished by passing responder cells over Sephadex G-10 prior to culture. The results suggest that the maturation process of CTL-precursor (CTL-P) cells into effector CTL in vitro depends upon proliferating T cells and accessory cells. [ABSTRACT FROM AUTHOR]
- Subjects :
- LYMPHOCYTES
T cells
LABORATORY mice
LYMPH nodes
LYMPHATICS
CELL-mediated cytotoxicity
Subjects
Details
- Language :
- English
- ISSN :
- 00192805
- Volume :
- 50
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 13403953