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PD-1 in human NK cells: evidence of cytoplasmic mRNA and protein expression.
- Source :
- OncoImmunology; 2019, Vol. 8 Issue 3, p1-1, 1p
- Publication Year :
- 2019
-
Abstract
- Under physiological conditions, PD-1/PD-L1 interactions regulate unwanted over-reactions of immune cells and contribute to maintain peripheral tolerance. However, in tumor microenvironment, this interaction may greatly compromise the immune-mediated anti-tumor activity. PD-1<superscript>+</superscript> NK cells have been detected in high percentage in peripheral blood and ascitic fluid of ovarian carcinoma patients. To acquire information on PD-1 expression and physiology in human NK cells, we analyzed whether PD-1 mRNA and protein are present in resting, surface PD-1<superscript>−</superscript>, NK cells from healthy donors. Both different splicing isoforms of PD-1 mRNA and a cytoplasmic pool of PD-1 protein were detected. Similar results were obtained also from both in vitro-activated and tumor-associated NK cells. PD-1 mRNA and protein were higher in CD56<superscript>dim</superscript> than in CD56<superscript>bright</superscript> NK cells. Confocal microscopy analyses revealed that PD-1 protein is present in virtually all NK cells analyzed. The present findings are compatible with a rapid surface expression of PD-1 in NK cells in response to appropriate, still undefined, stimuli. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 21624011
- Volume :
- 8
- Issue :
- 3
- Database :
- Complementary Index
- Journal :
- OncoImmunology
- Publication Type :
- Academic Journal
- Accession number :
- 134309798
- Full Text :
- https://doi.org/10.1080/2162402X.2018.1557030