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Real-time monitoring of T-cell-secreted interferon-γ for the diagnosis of tuberculosis.
- Source :
- Biotechnology & Biotechnological Equipment; Aug2018, Vol. 32 Issue 4, p993-998, 6p
- Publication Year :
- 2018
-
Abstract
- In this paper, we report the development of a label-free biosensor, based on surface plasmon resonance, for real-time monitoring of captured human CD4<superscript>+</superscript>T-cells, and their dynamic cytokine production upon specific antigen stimulation. Microarrays of CD4<superscript>+</superscript>T-cells, and interferon gamma (IFN-γ) specific antibody (Ab) spots were printed side by side onto the poly(OEGMA-co-HEMA) matrix. The placement of CD4<superscript>+</superscript> T-cells near the anti IFN-γ Ab-coated spots ensured a high local concentration of secreted IFN-γ for detection. We have demonstrated that this approach enables the detection of tuberculosis (TB) infection in clinical samples, with an overall sensitivity of 85.5% and an overall specificity of 97.7%. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 13102818
- Volume :
- 32
- Issue :
- 4
- Database :
- Complementary Index
- Journal :
- Biotechnology & Biotechnological Equipment
- Publication Type :
- Academic Journal
- Accession number :
- 134749130
- Full Text :
- https://doi.org/10.1080/13102818.2018.1432416