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Bufalin Induces Apoptotic Cell Death in Human Nasopharyngeal Carcinoma Cells through Mitochondrial ROS and TRAIL Pathways.
- Source :
- American Journal of Chinese Medicine; 2019, Vol. 47 Issue 1, p237-257, 21p, 2 Diagrams, 4 Graphs
- Publication Year :
- 2019
-
Abstract
- The aim of this study was to investigate the effects of bufalin on human nasopharyngeal carcinoma NPC-TW 076 cells in vitro. Bufalin is a cardiotonic steroid and a key active ingredient of the Chinese medicine ChanSu. The extracts of Chansu are used for various cancer treatments in China. In the present study, bufalin induced cell morphological changes, decreased total cell viability and induced G<subscript>2</subscript>/M phase arrest of cell cycle in NPC-TW 076 cells. Results also indicated that bufalin induced chromatin condensation (cell apoptosis) and DNA damage by DAPI staining and comet assay, respectively. The induced apoptotic cell death was further confirmed by annexin-V/PI staining assay. In addition, bufalin also increased ROS and Ca 2 + production and decreased the levels of Δ Ψ m . Furthermore, the alterations of ROS, ER stress and apoptosis associated protein expressions were investigated by Western blotting. Results demonstrated that bufalin increased the expressions of ROS associated proteins, including SOD (Cu/Zn), SOD2 (Mn) and GST but decreased that of catalase. Bufalin increased ER stress associated proteins (GRP78, IRE-1 α , IRE-1 β , caspase-4, ATF-6 α , Calpain 1, and GADD153). Bufalin increased the pro-apoptotic proteins Bax, and apoptotic associated proteins (cytochrome c, caspase-3, -8 and -9, AIF and Endo G) but reduced anti-apoptotic protein Bcl-2 in NPC-TW 076 cells. Furthermore, bufalin elevated the expressions of TRAIL-pathway associated proteins (TRAIL, DR4, DR5, and FADD). Based on these findings, we suggest bufalin induced apoptotic cell death via caspase-dependent, mitochondria-dependent and TRAIL pathways in human nasopharyngeal carcinoma NPC-TW 076 cells. [ABSTRACT FROM AUTHOR]
- Subjects :
- REACTIVE oxygen species
ANALYSIS of variance
ANTINEOPLASTIC agents
APOPTOSIS
BIOLOGICAL assay
CELL culture
CELLULAR signal transduction
DIMETHYL sulfoxide
FLOW cytometry
GLUTAMINE
HETEROCYCLIC compounds
PENICILLIN
CHINESE medicine
NASOPHARYNX cancer
PROTEINS
SERUM
STREPTOMYCIN
WESTERN immunoblotting
DATA analysis software
CELL survival
DESCRIPTIVE statistics
PHARMACODYNAMICS
Subjects
Details
- Language :
- English
- ISSN :
- 0192415X
- Volume :
- 47
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- American Journal of Chinese Medicine
- Publication Type :
- Academic Journal
- Accession number :
- 134771227
- Full Text :
- https://doi.org/10.1142/S0192415X19500125