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1,25(OH)2D3 regulates c-myc mRNA levels in tonsillar T lymphocytes.
- Source :
- Immunology; Dec91, Vol. 74 Issue 4, p589-593, 5p
- Publication Year :
- 1991
-
Abstract
- The effects of 1,25(OH)<subscript>2</subscript>D<subscript>3</subscript> on proliferation, c-myc mRNA levels and 1,25(OH)<subscript>2</subscript>D<subscript>3</subscript> receptor expression in activated tonsillar T lymphocytes were studied. Activation of resting T cells with phytohaemagglutinin (PHA) for 72 hr led to an increase in proliferation, c-myc mRNA levels and to induction of 1,25(OH)<subscript>2</subscript>D<subscript>3</subscript> receptor expression. However, when activation was carried out in the presence of 1,25(OH)<subscript>2</subscript>D<subscript>3</subscript>, there was inhibition of PHA-stimulated proliferation and c-myc mRNA levels. Increased cell proliferation, c-myc mRNA expression and 1,25(OH)<subscript>2</subscript>D<subscript>3</subscript> receptor number were also observed, albeit to a lesser extent, when T cells were stimulated by phorbol myristate acetate (PMA), anti-CD3 antibody or A23187. However, in these eases 1,25(OH)<subscript>2</subscript>D<subscript>3</subscript> was unable to prevent increased proliferation or c-myc mRNA expression. PMA and anti-CD3 used in combination produced similar or greater changes in proliferation, c-myc mRNA levels, 1,25(OH)<subscript>2</subscript>D<subscript>3</subscript> receptor expression and responsiveness to the hormone when compared to PHA alone. Thus the inhibition of c-myc expression in activated T lymphocytes by 1,25(OH)<subscript>2</subscript>D<subscript>3</subscript> can be related to its anti-proliferative effects. Moreover this inhibition seems to be dependent on the level of 1,25(OH)<subscript>2</subscript>D<subscript>3</subscript> receptor expression, which in turn appears to be related to the degree of cell activation. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00192805
- Volume :
- 74
- Issue :
- 4
- Database :
- Complementary Index
- Journal :
- Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 13487826