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T-cell activation. V. Anti-major histocompatibility complex class I antibody-induced activation and clonal abortion in Jurkat T-leukaemic cells.
- Source :
- Immunology; Mar1993, Vol. 78 Issue 3, p444-448, 5p
- Publication Year :
- 1993
-
Abstract
- We have studied activation-induced changes in intracellular calcium [Ca<superscript>2+</superscript> ]<subscript>1</subscript>, interleukin-2 (IL-2) secretion, and clonal abortion of the human leukaemic T-cell line Jurkat and three T-cell receptor (TcR)/CD3 receptor negative clones deficient for the TcRα, TcRβ and CD3γ chains respectively, as well as three transfectant clones reconstituted with the appropriate TcR/CD3 cDNA. For activation, the cells were exposed to anti-TcR/CD3, anti-CD2 and anti-major histocompatibility complex (anti-MHC) class I monoclonal antibodies (mAb) respectively. Cellular activation by these mAb leading to an increased IL-2 secretion was preceded by a rise in [Ca<superscript>2+</superscript>]<subscript>1</subscript> and was relatively dependent on the expression of the a TcR/CD3 complex. In contrast, anti-MHC class I mAb-induced clonal abortion in Jurkat T cells may occur without previous fluctuations in [Ca<superscript>2+</superscript>]<subscript>1</subscript> and appeared to be independent of TcR/CD3 expression. The present observations suggest the existence of different secondary messenger systems operating in Jurkat cells following activation via the TcR/CD3, CD2 and the MHC class I pathways, respectively. [ABSTRACT FROM AUTHOR]
- Subjects :
- CALCIUM in the body
INTERLEUKINS
CELL lines
T cell receptors
MONOCLONAL antibodies
Subjects
Details
- Language :
- English
- ISSN :
- 00192805
- Volume :
- 78
- Issue :
- 3
- Database :
- Complementary Index
- Journal :
- Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 13489274