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An allospecific murine T helper clone which can help both T and B cell responses <em>in vitro</em> and <em>in vivo</em>.
- Source :
- Immunology; May84, Vol. 52 Issue 1, p55-65, 11p
- Publication Year :
- 1984
-
Abstract
- Both B lymphocytes and cytotoxic T lymphocytes respond to signals from the T helper (T<subscript>h</subscript>) compartment, and such signals are mediated by a number of biochemically distinct factors. This raises the question whether help for B cells and T cells is a function of one or several different kinds of T<subscript>h</subscript> cell. Here we describe an in vitro and in vivo study of this problem, using a T<subscript>h</subscript> clone, designated MTH-1. The clone carries the cell surface markers Thy-1 and L3T4a, but lacks Lyt-2. It recognizes a minor alloantigen shared by DBA/2, B10.D2 and NZB spleen cells, and such recognition is restricted by H-2E<superscript>d</superscript>. Recognition of antigen in vitro is accompanied by secretion of IL-2. In vivo, both primary and secondary CTL responses to multiple minor alloantigens are enhanced by small numbers (≤10<superscript>4</superscript>) of MTH-1 cells. Recognition of alloantigen in a T-depleted B cell population results in the polyclonal activation and maturation of the B cells to secrete immunoglobulin; also, antigen-primed B ceils are augmented in their in vivo synthesis of specific antibody to the Thy-1·1 alloantigen by around 10<superscript>5</superscript> MTH-1 cells. Taken together, these results suggest a single T<subscript>h</subscript> clone can help both B cells and T cells. [ABSTRACT FROM AUTHOR]
- Subjects :
- B cells
T cells
LYMPHOCYTES
CELL differentiation
CELL membranes
IMMUNOGLOBULINS
Subjects
Details
- Language :
- English
- ISSN :
- 00192805
- Volume :
- 52
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 13506366