An allospecific murine T helper clone which can help both T and B cell responses <em>in vitro</em> and <em>in vivo</em>.

Both B lymphocytes and cytotoxic T lymphocytes respond to signals from the T helper (T_h) compartment, and such signals are mediated by a number of biochemically distinct factors. This raises the question whether help for B cells and T cells is a function of one or several different kinds of T_h cell. Here we describe an in vitro and in vivo study of this problem, using a T_h clone, designated MTH-1. The clone carries the cell surface markers Thy-1 and L3T4a, but lacks Lyt-2. It recognizes a minor alloantigen shared by DBA/2, B10.D2 and NZB spleen cells, and such recognition is restricted by H-2E^d. Recognition of antigen in vitro is accompanied by secretion of IL-2. In vivo, both primary and secondary CTL responses to multiple minor alloantigens are enhanced by small numbers (≤10⁴) of MTH-1 cells. Recognition of alloantigen in a T-depleted B cell population results in the polyclonal activation and maturation of the B cells to secrete immunoglobulin; also, antigen-primed B ceils are augmented in their in vivo synthesis of specific antibody to the Thy-1·1 alloantigen by around 10⁵ MTH-1 cells. Taken together, these results suggest a single T_h clone can help both B cells and T cells. [ABSTRACT FROM AUTHOR]