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A Systematic Method for Evaluating Quality of Jinqi Jiangtang Tablet: Simultaneous Determination of 21 Compounds by HPLC-MS.
- Source :
- Chromatographia; Feb2019, Vol. 82 Issue 2, p605-613, 9p
- Publication Year :
- 2019
-
Abstract
- Jinqi Jiangtang tablet (JQJTT), a traditional Chinese herbal medicine for treating diabetes, consists of Coptis chinensis, Astragalus membranaceus, and Lonicera japonica. In this study, we developed a method using high-performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry (HPLC-MS) to analyze 21 compounds in JQJTT, belonging to five different types. Separation was performed on a C<subscript>18</subscript> column (150 × 4.6 mm, 5 µm), and the mobile phase for the gradient elution consisted of 2 mM ammonium acetate and 0.1% acetic acid as an aqueous phase and acetonitrile as an organic phase. The method showed good linearity (r > 0.9954). The relative standard deviation (RSD) values for the intra- and inter-day precision were less than 4.98% and 4.93%, respectively. The recovery at three concentration levels ranged from 90.15 to 109.90%, with RSD below 4.69%. Sample solutions were proven to be stable for more than 24 h at 4 °C (RSD < 4.87%). The novel method was successfully applied to systematic quality evaluation of JQJTT based on the quantification of the 21 compounds. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00095893
- Volume :
- 82
- Issue :
- 2
- Database :
- Complementary Index
- Journal :
- Chromatographia
- Publication Type :
- Academic Journal
- Accession number :
- 135085752
- Full Text :
- https://doi.org/10.1007/s10337-018-3667-1