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Metabolic Adjustment of Arabidopsis Root Suspension Cells During Adaptation to Salt Stress and Mitotic Stress Memory.

Authors :
Chun, Hyun Jin
Baek, Dongwon
Cho, Hyun Min
Jung, Hyun Suk
Jeong, Myeong Seon
Jung, Wook-Hun
Choi, Cheol Woo
Lee, Su Hyeon
Jin, Byung Jun
Park, Mi Suk
Kim, Hyun-Jin
Chung, Woo Sik
Lee, Sang Yeol
Bohnert, Hans J
Bressan, Ray A
Yun, Dae-Jin
Hong, Young-Shick
Kim, Min Chul
Source :
Plant & Cell Physiology; Mar2019, Vol. 60 Issue 3, p612-625, 14p
Publication Year :
2019

Abstract

Sessile plants reprogram their metabolic and developmental processes during adaptation to prolonged environmental stresses. To understand the molecular mechanisms underlying adaptation of plant cells to saline stress, we established callus suspension cell cultures from Arabidopsis roots adapted to high salt for an extended period of time. Adapted cells exhibit enhanced salt tolerance compared with control cells. Moreover, acquired salt tolerance is maintained even after the stress is relieved, indicating the existence of a memory of acquired salt tolerance during mitotic cell divisions, known as mitotic stress memory. Metabolite profiling using <superscript>1</superscript>H-nuclear magnetic resonance (NMR) spectroscopy revealed metabolic discrimination between control, salt-adapted and stress-memory cells. Compared with control cells, salt-adapted cells accumulated higher levels of sugars, amino acids and intermediary metabolites in the shikimate pathway, such as coniferin. Moreover, adapted cells acquired thicker cell walls with higher lignin contents, suggesting the importance of adjustments of physical properties during adaptation to elevated saline conditions. When stress-memory cells were reverted to normal growth conditions, the levels of metabolites again readjusted. Whereas most of the metabolic changes reverted to levels intermediate between salt-adapted and control cells, the amounts of sugars, alanine, γ-aminobutyric acid and acetate further increased in stress-memory cells, supporting a view of their roles in mitotic stress memory. Our results provide insights into the metabolic adjustment of plant root cells during adaptation to saline conditions as well as pointing to the function of mitotic memory in acquired salt tolerance. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
00320781
Volume :
60
Issue :
3
Database :
Complementary Index
Journal :
Plant & Cell Physiology
Publication Type :
Academic Journal
Accession number :
135109347
Full Text :
https://doi.org/10.1093/pcp/pcy231